Viral Recombinant Proteins

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Simultaneous Administration of Recombinant Measles Viruses Expressing Respiratory Syncytial Virus Fusion (F) and Nucleo (N) Proteins Induced Humoral and Cellular Immune Responses in Cotton Rats.

We previously reported that measles virus expressing recombinant respiratory syncytial virus (RSV) fusion protein (F), MVAIK / RSV / F, induced neutralizing antibodies against RSV, and they express RSV-NP (MVAIK / RSV / NP) and M2-1 ( MVAIK / RSV / M2-1) RSV-specific induced CD8⁺ / cell IFN-γ⁺, but no neutralizing antibodies. In this study, MVAIK / RSV / F and MVAIK / RSV / NP simultaneously given to the cotton rat and Viral Recombinant Proteins immune responses and protective effects compared to MVAIK / RSV / F only. sufficient neutralizing antibodies against RSV and RSV-specific CD8⁺ / IFN-γ⁺ cells were observed after re-immunization with simultaneous administration. After the RSV challenge, CD8⁺ / IFN-γ⁺ increased in spleen cells obtained from immunization groups simultaneously in response to F and NP peptide. amount to more than CD8⁺ / IFN-γ⁺ and CD4⁺ / IFN-γ⁺ cells were detected in the lung tissue of simultaneous immunization group after RSV challenge. There is no RSV ...

Efficient Production of Human Norovirus-Specific IgY in Egg Yolks by Vaccination of Hens with a Recombinant Vesicular Stomatitis Virus Expressing VP1 Protein.

Human norovirus (HuNoV) is responsible for over 95% of outbreaks of acute nonbacterial gastroenteritis worldwide. Despite great efforts, there is no vaccine or effective therapeutic intervention against this virus. Chicken immunoglobulin Y (IgY) based passive immunization has proven to be an effective strategy for preventing and treating many diseases enteric viruses. Here, we develop highly efficient bioreactor Parasite Recombinant Proteins to produce high titers of specific HuNoV yellow chicken IgY in using recombinant vesicular stomatitis virus expressing HuNoV capsid protein (rVSV-VP1) as antigen. We first show that HuNoV VP1 protein is highly expressed in chicken cells infected with rVSV-VP1. Furthermore, we find that the White Leghorn chickens were immunized intramuscularly with rVSV-VP1 high level trigger specific HuNoV yolk antibody IgY. yellow efficiently purified IgY recognized by HuNoV virus-like particle (VLP). Importantly, specific IgY efficient HuNoV HuNoV blo...

Development of an enzyme-linked immunosorbent assay using recombinant protein antigen for the diagnosis of Chikungunya virus.

We describe here the development of in-house enzyme linked immunosorbent assay (ELISA) for diagnostic Chikungunya virus (CHIKV) infection using a recombinant protein of CHIKV. Recombinant protein gene was designed based on 154 sequences and we used computational methods to predict the structure and antigenic potential. For the prediction of confirmation, the Monkey Recombinant Proteins gene coding for a recombinant protein CHIKV (rCHIKVp) has been synthesized and expressed in a prokaryotic system. Subsequently, the protein was purified by affinity chromatography and used as an antigen in an indirect ELISA. We present data on production optimization and preparation of the recombinant antigen ELISA to detect IgG against CHIKV in human sera. Development of an enzyme-linked immunosorbent assay for viral infections in horses latex using recombinant E2 protein as an antigen. Gum virus causes fever, skin eruptions, and limb edema in horses. For high-throughput and time-saving meth...

Optimization of recombinant Zika virus NS1 protein secretion from HEK293 cells.

Sensitive, accurate and cost-effective diagnostic tests are needed to detect the Zika virus (ZIKV) infection. Nonstructural 1 (NS1) glycoprotein is an excellent diagnostic marker because it was released in a hexameric conformation of the infected cells into the bloodstream of patients in the early course of infection. We established a stable system Fungal Recombinant Proteins of his rZNS1-expression in HEK293 cells through lentiviral transduction. New optimization approach to improve his rZNS1 secretion of proteins in mammalian expression systems is achieved through incubation of 50 nM rapamycin followed by incubation in serum-free media for 9 days, attaining the protein from ~ 10 mg / l of culture medium. His rZNS1 purified hexamer recognized by anti-NS1 antibodies in serum ZIKV patients, and demonstrated the ability to induce a humoral response in mice immunized. recombinant proteins obtained are reliable biological tool that can potentially be applied in the develo...

Recombinant Lactococcus Lactis Expressing M1-HA2 Fusion Protein Provides Protective Mucosal Immunity Against H9N2 Avian Influenza Virus in Chickens.

H9N2 subtype avian influenza virus of low pathogenicity (LPAIV) are distributed worldwide and cause enormous economic losses in the poultry industry. Although almost all the chickens immunized with the inactivated vaccine, the disease is still widespread. We speculate that increase mucosal or cellular immune response may contribute to improving the H9N2 virus surveillance. In this study, we Bovine Recombinant Proteins constructed a novel Lactococcus lactis (L. lactis) strain expressing a recombinant fusion protein consisting of the M1 and HA2 proteins derived from the H9N2 viral strain endemic conserved antigens. M1-HA2 fusion protein was cloned downstream of the gene encoding the secretory peptide, and we subsequently confirmed that the fusion protein is secreted from L. lactis by Western blotting. We assess the immunogenicity and protective effects of recombinant L. lactis this tension. Eight chicken 1-day-old were divided into four groups, and the experimental grou...