Optimization of recombinant Zika virus NS1 protein secretion from HEK293 cells.

Sensitive, accurate and cost-effective diagnostic tests are needed to detect the Zika virus (ZIKV) infection. Nonstructural 1 (NS1) glycoprotein is an excellent diagnostic marker because it was released in a hexameric conformation of the infected cells into the bloodstream of patients in the early course of infection.

 We established a stable system Fungal Recombinant Proteins of his rZNS1-expression in HEK293 cells through lentiviral transduction. New optimization approach to improve his rZNS1 secretion of proteins in mammalian expression systems is achieved through incubation of 50 nM rapamycin followed by incubation in serum-free media for 9 days, attaining the protein from ~ 10 mg / l of culture medium. 

His rZNS1 purified hexamer recognized by anti-NS1 antibodies in serum ZIKV patients, and demonstrated the ability to induce a humoral response in mice immunized. recombinant proteins obtained are reliable biological tool that can potentially be applied in the development of diagnostic tests to detect infected ZIKV in patients during the acute phase.

Gold nanoparticles enhance the immune response in mice against hog cholera virus E2 recombinant proteins.

To create a novel subunit vaccines that AuNPs used as a carrier to enhance the immune response in mice against recombinant classical swine fever virus E2 protein (CSFV E2) .Gold nanoparticles (AuNPs) successfully merged into the E2 protein forms a complex and unstable particle called conjugated AuNPs E2 (E2 -AuNPs). 

In vitro studies have shown that Hapten Conjugates Proteins E2-AuNPs complex have the same immunogenicity of protein E2, and AuNPs can promote phagocytosis of E2 protein by antigen-presenting cells (APC). In vivo results of BALB / c mice showed that antibody levels, the index of lymphocyte proliferation, IFN-γ and IL-10 cytokines induced by E2-AuNPs is relatively higher than E2 or AuNPs group.

This findings demonstrate the potential of using AuNPs as a carrier to improve immune response to develop CSFV subunit vaccine. This model also contributes to the development of subunit flavivirus vaccines, such as hepatitis C virus (HCV) and bovine viral diarrhea virus (BVDV).