Development of an enzyme-linked immunosorbent assay using recombinant protein antigen for the diagnosis of Chikungunya virus.
We describe here the development of in-house enzyme linked immunosorbent assay (ELISA) for diagnostic Chikungunya virus (CHIKV) infection using a recombinant protein of CHIKV. Recombinant protein gene was designed based on 154 sequences and we used computational methods to predict the structure and antigenic potential. For the prediction of confirmation, the Monkey Recombinant Proteins gene coding for a recombinant protein CHIKV (rCHIKVp) has been synthesized and expressed in a prokaryotic system. Subsequently, the protein was purified by affinity chromatography and used as an antigen in an indirect ELISA. We present data on production optimization and preparation of the recombinant antigen ELISA to detect IgG against CHIKV in human sera. Development of an enzyme-linked immunosorbent assay for viral infections in horses latex using recombinant E2 protein as an antigen. Gum virus causes fever, skin eruptions, and limb edema in horses. For high-throughput and time-saving meth...