Recombinant Lactococcus Lactis Expressing M1-HA2 Fusion Protein Provides Protective Mucosal Immunity Against H9N2 Avian Influenza Virus in Chickens.
H9N2 subtype avian influenza virus of low pathogenicity (LPAIV) are distributed worldwide and cause enormous economic losses in the poultry industry. Although almost all the chickens immunized with the inactivated vaccine, the disease is still widespread. We speculate that increase mucosal or cellular immune response may contribute to improving the H9N2 virus surveillance. In this study, we Bovine Recombinant Proteins constructed a novel Lactococcus lactis (L. lactis) strain expressing a recombinant fusion protein consisting of the M1 and HA2 proteins derived from the H9N2 viral strain endemic conserved antigens. M1-HA2 fusion protein was cloned downstream of the gene encoding the secretory peptide, and we subsequently confirmed that the fusion protein is secreted from L. lactis by Western blotting. We assess the immunogenicity and protective effects of recombinant L. lactis this tension. Eight chicken 1-day-old were divided into four groups, and the experimental grou...