Efficient Production of Human Norovirus-Specific IgY in Egg Yolks by Vaccination of Hens with a Recombinant Vesicular Stomatitis Virus Expressing VP1 Protein.
Human norovirus (HuNoV) is responsible for over 95% of outbreaks of acute nonbacterial gastroenteritis worldwide. Despite great efforts, there is no vaccine or effective therapeutic intervention against this virus. Chicken immunoglobulin Y (IgY) based passive immunization has proven to be an effective strategy for preventing and treating many diseases enteric viruses. Here, we develop highly efficient bioreactor Parasite Recombinant Proteins to produce high titers of specific HuNoV yellow chicken IgY in using recombinant vesicular stomatitis virus expressing HuNoV capsid protein (rVSV-VP1) as antigen. We first show that HuNoV VP1 protein is highly expressed in chicken cells infected with rVSV-VP1. Furthermore, we find that the White Leghorn chickens were immunized intramuscularly with rVSV-VP1 high level trigger specific HuNoV yolk antibody IgY. yellow efficiently purified IgY recognized by HuNoV virus-like particle (VLP). Importantly, specific IgY efficient HuNoV HuNoV blo...