Efficient Production of Human Norovirus-Specific IgY in Egg Yolks by Vaccination of Hens with a Recombinant Vesicular Stomatitis Virus Expressing VP1 Protein.

Human norovirus (HuNoV) is responsible for over 95% of outbreaks of acute nonbacterial gastroenteritis worldwide. Despite great efforts, there is no vaccine or effective therapeutic intervention against this virus. Chicken immunoglobulin Y (IgY) based passive immunization has proven to be an effective strategy for preventing and treating many diseases enteric viruses.

Here, we develop highly efficient bioreactor Parasite Recombinant Proteins to produce high titers of specific HuNoV yellow chicken IgY in using recombinant vesicular stomatitis virus expressing HuNoV capsid protein (rVSV-VP1) as antigen. We first show that HuNoV VP1 protein is highly expressed in chicken cells infected with rVSV-VP1. 

Furthermore, we find that the White Leghorn chickens were immunized intramuscularly with rVSV-VP1 high level trigger specific HuNoV yolk antibody IgY. yellow efficiently purified IgY recognized by HuNoV virus-like particle (VLP). Importantly, specific IgY efficient HuNoV HuNoV blocked VLP binding to all three types (A, B, and O) histo-blood group antigens (HBGAs), factors of attachment to HuNoV.

 In addition, the receptor blocking activity of IgY remain stable at temperatures below 70 ° C and at a pH range from 4 to 9. Thus, the chickens immunized with VSV-VP1 can be cost-effective and practical strategies for large-scale production of antibody IgY-HuNoV anti for potential use as a prophylactic treatment and therapy against infections HuNoV.

Generation and evaluation of goose origin expressing recombinant Newcastle disease virus proteins Cap avastrovirus goose origin as a bivalent vaccine in goslings.

Currently, the two geese astrovirus (GoAstV) and goose-origin Newcastle disease virus (NDV) widespread infectious agent for the goose. There is no vaccine for GoAstV. Capsid proteins can induce neutralizing antibodies human astroviruses (HAstV). molecular analysis of genomic region encoding the capsid protein (ORF2) of geese astrovirus has revealed that it contains a neutralization epitope. Goose-origin NDV is also an infectious agent.

 Various existing NDV strains which can be commonly used as a vaccine vector. In this study, a fusion protein cleavage site in the F ↓ RRQKR backbone of goose-origin virulent NDV SH-12 is converted into motive GRQGR avirulent L. ↓ goose-origin modified NDV expressing recombinant vaccine virus capsid protein (Cap) of GoAstV generated as bivalent vaccine using reverse-genetics approach. 

Recombinant virus, rNDV / GoAstV-Cap, the attenuated Purified Recombinant Proteins and the same growth dynamics, cytopathic effects, and viral titers in vitro preserved when compared with the Lasota strain. GoAstV-Cap protein expression in rNDV / GoAstV-Cap-infected cells was detected by immunofluorescence assay and Western blotting. Goslings inoculated with rNDV / GoAstV-Cap showed obvious signs of disease and causes the immune response and serum antibody responses to NDV-specific GoAstV-Cap-specific vaccination to control Lasota.