Simultaneous Administration of Recombinant Measles Viruses Expressing Respiratory Syncytial Virus Fusion (F) and Nucleo (N) Proteins Induced Humoral and Cellular Immune Responses in Cotton Rats.

We previously reported that measles virus expressing recombinant respiratory syncytial virus (RSV) fusion protein (F), MVAIK / RSV / F, induced neutralizing antibodies against RSV, and they express RSV-NP (MVAIK / RSV / NP) and M2-1 ( MVAIK / RSV / M2-1) RSV-specific induced CD8⁺ / cell IFN-γ⁺, but no neutralizing antibodies. In this study, MVAIK / RSV / F and MVAIK / RSV / NP simultaneously given to the cotton rat and Viral Recombinant Proteins immune responses and protective effects compared to MVAIK / RSV / F only. sufficient neutralizing antibodies against RSV and RSV-specific CD8⁺ / IFN-γ⁺ cells were observed after re-immunization with simultaneous administration.

 After the RSV challenge, CD8⁺ / IFN-γ⁺ increased in spleen cells obtained from immunization groups simultaneously in response to F and NP peptide. amount to more than CD8⁺ / IFN-γ⁺ and CD4⁺ / IFN-γ⁺ cells were detected in the lung tissue of simultaneous immunization group after RSV challenge. There is no RSV was detected were recovered from lung homogenates in groups immunized. mild inflammatory reaction with broncho-epithelial cell thickening and infiltration of inflammatory cells were observed in lung tissue obtained from cotton rats immunized with MVAIK / RSV / F alone after RSV challenge.

 No inflammatory response was observed after RSV challenge on simultaneous immunization group. These results indicate that the combined administration with MVAIK / RSV / F and MVAIK / RSV / NP induces humoral and cellular immune responses and demonstrate effective protection against RSV, showed the importance of cellular immunity.

The Perennial Use of Green Fluorescent Protein Marker Live vaccinia virus Ankara Recombinant acute Runway Shows No Adverse reactions in Mice.

recombinant viral vector is a promising strategy for vaccine research. Among the viral vectors are available, members of the family Poxviridae-especially modified vaccinia virus Ankara (MVA) -Stand as immunogenic and safe vaccine platform. Because MVA usually do not produce plaques in cell culture, selection markers look like green fluorescent protein (GFP) is often incorporated into the construction to facilitate the recognition of recombinants. 

However, genetic markers must be removed prior to clinical trials. Here, we evaluate the acute response produced in mice immunized with MVA vector Yeast Recombinant Proteins in which the GFP marker is removed. We observed no difference in neutrophils, monocytes, or total recruitment of leukocytes between animals inoculated with MVA or MVA-GFP.

Likewise, there was no difference in neutrophil activation between groups of rats. liver function was not altered either MVA or MVA-GFP-inoculated mice, and we observed no histopathological changes in different networks of virus-inoculated animals