Sensitive, accurate and cost-effective diagnostic tests are needed to detect the Zika virus (ZIKV) infection. Nonstructural 1 (NS1) glycoprotein is an excellent diagnostic marker because it was released in a hexameric conformation of the infected cells into the bloodstream of patients in the early course of infection. We established a stable system Fungal Recombinant Proteins of his rZNS1-expression in HEK293 cells through lentiviral transduction. New optimization approach to improve his rZNS1 secretion of proteins in mammalian expression systems is achieved through incubation of 50 nM rapamycin followed by incubation in serum-free media for 9 days, attaining the protein from ~ 10 mg / l of culture medium. His rZNS1 purified hexamer recognized by anti-NS1 antibodies in serum ZIKV patients, and demonstrated the ability to induce a humoral response in mice immunized. recombinant proteins obtained are reliable biological tool that can potentially be applied in the develo...